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KMID : 0624620180510060290
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BMB Reports
2018 Volume.51 No. 6 p.290 ~ p.295
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Investigation of function and regulation of the YB-1 cellular factor in HIV replication
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Jung Yu-Mi
Yu Kyung-Lee
Park Seong-Hyun
Lee Seong-Deok
Kim Min-Jeong
You Ji-Chang
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Abstract
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Y-box binding protein 1 (YB-1) is a member of the cold-shock domain (CSD) protein superfamily. It participates in a wide variety of cellular events, including transcription, RNA splicing, translation, DNA repair, drug resistance, and stress responses. We investigated putative functions of YB-1 in HIV-1 replication. Functional studies using overexpression or knockdown of YB-1 in conjunction with transfection of proviral DNA showed that YB-1 enhances virus production. We found YB-1 regulates HIV-1 production by stimulating viral transcription using HIV-1 LTR sequence U3RU5 with Luciferase assay. We also identified a specific region from amino acids 1 to 324 of YB-1 as necessary for the participation of the protein in the production of virions.
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KEYWORD
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HIV-1, HIV-1 LTR, HIV-1 promoter, Transcription activation, YB-1
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